Determination of tortoside A in Ilicis pubescentis by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3146-3148, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-251178
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.</p><p><b>METHOD</b>Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (1783), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT:
Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.</p><p><b>CONCLUSION</b>It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chemistry
/
Chromatography, High Pressure Liquid
/
Ilex
/
Glycosides
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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