Identification of Schisandra sphenanthera and S. chinensis by random amplified polymorphic DNA sequence characterized applied region / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3083-3085, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-251191
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new method for the identification of Schisandra sphenanthera and S. chinensis.</p><p><b>METHOD</b>Random amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers.</p><p><b>RESULT</b>Screening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region.</p><p><b>CONCLUSION</b>RAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Random Amplified Polymorphic DNA Technique
/
Schisandra
/
Genetics
/
Methods
Type of study:
Controlled clinical trial
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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