Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase
Experimental & Molecular Medicine
;
: 187-192, 2000.
Article
in English
| WPRIM
| ID: wpr-25125
ABSTRACT
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
HeLa Cells
/
Exons
/
Alternative Splicing
/
DNA, Complementary
/
Reverse Transcriptase Polymerase Chain Reaction
/
Lysosomes
/
Mannosidases
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2000
Type:
Article
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