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Thymic stromal lmphopoietin pomotes macrophage-derived foam cell formation / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 23-28, 2014.
Article in English | WPRIM | ID: wpr-251366
ABSTRACT
The effect of thymic stromal lymphopoietin (TSLP) on macrophage-derived foam cell formation and the underlying mechanism were studied. Macrophages isolated from C57BL/6 mice were co-cultured in vitro with different concentrations of TSLP or TSLPR-antibody in the presence of oxidized low density lipoprotein (ox-LDL). The effects of TSLP on macrophage-derived foam cell formation were observed by using oil red O staining and intracellular lipid determination. The expression levels of foam cell scavenger receptors (CD36 and SRA) as well as ABCA1 and TSLPR were detected by using RT-PCR and Western blotting. As compared with the control group, TSLP treatment significantly promoted lipid accumulation in macrophages, significantly increased protein expression of CD36 and TSLPR in a dose-dependent manner, and significantly reduced the expression of ABCA1 protein in a dose-dependent manner. No significant differences were noted between the TSLPR-antibody group and the control group. TSLP may down-regulate the expression of cholesterol efflux receptor ABCA1 and up-regulate scavenger receptor expression via the TSLPR signaling pathway, thereby promoting macrophage-derived foam cell formation.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Immunoglobulins / Gene Expression / Cells, Cultured / Blotting, Western / Cholesterol / Cholesterol Esters / Cytokines / Receptors, Cytokine / CD36 Antigens Limits: Animals Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Immunoglobulins / Gene Expression / Cells, Cultured / Blotting, Western / Cholesterol / Cholesterol Esters / Cytokines / Receptors, Cytokine / CD36 Antigens Limits: Animals Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article