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Impact of probiotics on toll-like receptor 4 expression in an experimental model of ulcerative colitis / 华中科技大学学报(医学)(英德文版)
Article in En | WPRIM | ID: wpr-251413
Responsible library: WPRO
ABSTRACT
Toll-like receptors (TLRs) are key components of the innate immune system which trigger antimicrobial host defense responses. This study aimed to investigate the impact of probiotics (Lactobacillus, Bifidobacterium) on the expression of TLR4 and tumor necrosis factor-alpha (TNF-α) in the colon mucosa of rat experimental ulcerative colitis model induced by trinitrobenzene sulfonic acid (TNBS)/ethanol and immune complexes. The gross and histological changes of the colonic mucosa were observed and assessed by the means-standard deviation and independent samples t-test. The protein expression levels of TLR4 and TNF-α were detected by using immunohistochemistry and Western blotting, respectively. It was revealed that there was visible infiltration of inflammatory cells, formation of crypt abscess, and the reduction of goblet cells in the colon tissue of experimental models. As compared with the control group, the levels of TLR4 and TNF-α protein were significantly increased in the model group (P<0.01 for both). No significant difference was found in the expression of TLR4 and TNF-α between the two-week probiotics treatment group and the model group (P>0.05), whereas significant reductions were shown in rats which were treated with probiotics for four weeks as compared with the model group (P<0.01). There was no significant difference between two probiotics-treated groups. Our results implied that probiotics were likely to play a key role in protecting ulcerative colitis by reducing the inflammatory factor TNF-α expression through inhibiting the TLR4 expression in the colon tissue of experimental models.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pharmacology / Physiology / Time Factors / Trinitrobenzenesulfonic Acid / Bifidobacterium / Immunohistochemistry / Colitis, Ulcerative / Blotting, Western / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley Type of study: Prognostic_studies Limits: Animals Language: En Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2013 Type: Article
Full text: 1 Index: WPRIM Main subject: Pharmacology / Physiology / Time Factors / Trinitrobenzenesulfonic Acid / Bifidobacterium / Immunohistochemistry / Colitis, Ulcerative / Blotting, Western / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley Type of study: Prognostic_studies Limits: Animals Language: En Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2013 Type: Article