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Silencing endothelin-3 expression attenuates the malignant behaviors of human melanoma cells by regulating SPARC levels / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 581-586, 2013.
Article in English | WPRIM | ID: wpr-251428
ABSTRACT
Endothelin-3 (ET-3) is aberrantly expressed in both metastatic melanoma tissues and cultured melanoma cells. Our previous work showed that ET-3 could promote survival of metastatic melanoma cells via its altered expression. In this study, we investigated the mechanisms responsible for these gene-induced phenotypes in melanoma cells. An ET-3 gene sequence-specific shRNA vector pLVTHM-ET3-RNAi was constructed and transfected into human malignant melanoma cells A375 and MMRU, and the resultant molecular events and cellular changes were examined. As compared with the empty-vector group, cell proliferation was slowed down, and the growth inhibition rates were 38.9% in A375 cells and 38.4% in MMRU cells after transfection. In addition, cell invasion capability was also inhibited, with a reduction of 62.2% in A375 cells and 54.3% in MMRU cells. The percentage of apoptotic cells was found to increase. Meanwhile, in both cell lines, secreted protein acidic and rich in cysteine (SPARC) levels were down-regulated together with inhibition of its upstream signaling molecule, NF-κB. Thus, the current results suggested that down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC and NF-κB.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Osteonectin / Endothelin-3 / Gene Silencing / Cell Line, Tumor / Genetics / Melanoma Limits: Humans Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Osteonectin / Endothelin-3 / Gene Silencing / Cell Line, Tumor / Genetics / Melanoma Limits: Humans Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2013 Type: Article