Characterization and comparison of interferon reference standards using UPLC-MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 75-80, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-251814
ABSTRACT
The study aims to characterize and compare interferon reference standards from 5 manufacturers. By testing molecular mass and trypsin-digested peptide mass mapping, the amino acid sequence was verified and post-translational modifications such as disulfide bond were identified. Results show that the molecular mass and amino acid sequence were consistent with theory; the disulfide bonds of 4 lots of interferon were Cys1-Cys98/Cys29-Cys138, 1 lot was Cys29-Cys139/Cys86-Cys99; N-terminal "+Met", acetyl N-terminal and Met oxidation were identified in part of the sample. UPLC-MS can be used to characterize and compare interferon reference standards from different manufacturers.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Reference Standards
/
Mass Spectrometry
/
Peptide Mapping
/
Protein Processing, Post-Translational
/
Chromatography, High Pressure Liquid
/
Amino Acid Sequence
/
Interferons
/
Methods
/
Molecular Weight
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article
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