Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 817-824, 2006.
Article
in English
| WPRIM
| ID: wpr-251850
ABSTRACT
The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Periosteum
/
Polyglycolic Acid
/
Polymers
/
Stem Cells
/
Biocompatible Materials
/
Bone Development
/
Cell Differentiation
/
Cell Survival
/
Cells, Cultured
Type of study:
Risk factors
Limits:
Humans
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2006
Type:
Article
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