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The effects of stem cell factor on proliferation, transmigration, capillary tube formation of endothelial cells and on the chemotaxis of CD133(+) cells / 中华血液学杂志
Chinese Journal of Hematology ; (12): 326-330, 2011.
Article in Chinese | WPRIM | ID: wpr-251963
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of stem cell factor (SCF) on proliferation, transmigration, capillary tube formation of human umbilical vein endothelial cells (HUVEC) and on the chemotaxis of CD133(+) cells.</p><p><b>METHODS</b>In the presence of blank control, SCF, vascular endothelial growth factor (VEGF), anti-human SCF (anti-SCF) or human IgG, the difference in proliferation capacity of HUVEC was analyzed by MTT and CCK-8 methods, and wound scratch assay and three-dimensional in vitro Matrigel assay were used for transmigration and capillary tube formation of HUVEC, respectively. In addition, the chemotaxis of CD133(+) cells sorted from human umbilical cord blood by flow cytometry was investigated by Transwell migration assay.</p><p><b>RESULTS</b>SCF didn't improve the proliferative capacity of HUVEC, but significantly enhanced the transmigration capacity, and increased capillary tube formation in a dose-dependent manner. The number of intact tubules [(30.0 ± 3.4)/10(5) HUVEC] formed by HUVECs in the presence of the optimal concentration of SCF (100 ng/ml) was remarkably higher than that in blank control group [(5.0 ± 2.6)/10(5) HUVEC, P < 0.01]. SCF also significantly induced a chemotactic response of CD133(+) cells, the transmembrane migration cell number into Transwell lower chamber was significantly higher in SCF group [(118.0 ± 6.5)/10(4) CD133(+) cells] than in blank control group [(47.0 ± 4.7)/10(4) CD133(+) cells, P < 0.01 ].</p><p><b>CONCLUSIONS</b>SCF significantly promotes the transmigration and capillary tube formation of HUVEC, and induces a chemotactic response of CD133(+) cells. SCF/c-kit signaling possibly plays a critical role in regulating angiogenesis of vascular endothelial cells and vasculogenesis of endothelial progenitor cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Sincalide / Cell Movement / Cells, Cultured / Stem Cell Factor / Neovascularization, Physiologic / Cell Biology / Cell Proliferation / Human Umbilical Vein Endothelial Cells / Metabolism Limits: Humans Language: Chinese Journal: Chinese Journal of Hematology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Sincalide / Cell Movement / Cells, Cultured / Stem Cell Factor / Neovascularization, Physiologic / Cell Biology / Cell Proliferation / Human Umbilical Vein Endothelial Cells / Metabolism Limits: Humans Language: Chinese Journal: Chinese Journal of Hematology Year: 2011 Type: Article