Detection of methylation levels of multi-genes by real-time PCR in patients with myelodysplastic syndrome / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 254-258, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-251981
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the promoter methylation levels of p15, CDH1, DAPK and HICI genes of patients with myelodysplastic syndrome (MDS) and explore the relationship between the level of methylation and clinical features.</p><p><b>METHODS</b>DNA methylation levels of p15, CDH1, DAPK and HICI in peripheral blood (PB) or bone marrow (BM) samples from 52 MDS patients were detected by real-time quantitative PCR. The correlation of the methylation level with clinical features and hematological findings was analyzed. 38 de novo AML patients and 46 normal individuals served as controls.</p><p><b>RESULTS</b>The methylation levels of p15, CDH1, DAPK and HICI were 16.23 ± 21.69, 6.59 ± 9.39, 0.14 ± 0.11 and 7.81 ± 9.70 in BM, and 14.96 ± 20.16, 6.00 ± 9.26, 0.12 ± 0.14 and 6.74 ± 9.72 in PB, respectively from 18 MDS patients, and the difference between BM and PB was not statistically significant (P > 0.05). The methylation levels of p15 (14.70 ± 18.17) and CDH1 (6.61 ± 8.79) genes in high risk (RAEBI/II) MDS were significantly higher than in low risk (RCMD/RARS/5q-, p15 1.99 ± 1.59, CDH1 1.23 ± 1.14 and RCMD, p15 3.02 ± 3.42, CDH11.53 ± 2.06) MDS or control (p15 1.69 ± 1.82, CDH1 1.01 ± 1.12) (P < 0.05). The methylation levels of DAPK gene had no difference among subtypes of MDS, and that of HIC1 gene only differed between RAEB I/II (9.16 ± 11.95) and control (2.49 ± 2.26) (P = 0.042). The difference of methylation levels of p15, CDH1, DAPK and HICI in BM was statistically significant among subtypes of MDS (P = 0.001, 0.003, 0.039, 0.023, respectively). And so did of p15 and DAPK in PB (P = 0.013, 0.006, respectively). The methylation level of p15 and CDH1 was significantly correlated with IPSS classification and blasts percentage in BM.</p><p><b>CONCLUSIONS</b>p15 and CDH1 genes are special hypermethylation genes in MDS. Methylation level of HIC1 gene showed an upward tendency from low risk to high risk MDS.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Myelodysplastic Syndromes
/
Cadherins
/
Case-Control Studies
/
Promoter Regions, Genetic
/
Calcium-Calmodulin-Dependent Protein Kinases
/
DNA Methylation
/
Apoptosis Regulatory Proteins
/
Cyclin-Dependent Kinase Inhibitor p15
/
Kruppel-Like Transcription Factors
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Observational study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS