Matrine inhibits the proliferation of neuroblastoma LA-N-5 cell and MYCN mRNA expression / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 225-227, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-252122
ABSTRACT
<p><b>OBJECTIVE</b>Neuroblastoma is the most common malignant solid tumor in children under 4 years. Amplification of MYCN oncogene is associated with advanced-stage disease, rapid tumor progression, resistance to treatment, and poor outcome. Matirne has the anti-tumor activity. This study was designed to investigate the effects of matrine on LA-N-5 cell line proliferation and MYCN gene mRNA expression.</p><p><b>METHODS</b>Neuroblastoma LA-N-5 cells were treated by 0.25, 0.50, 0.75 or 1.00 mg/mL matrine. MTT was used to measure the levels of the proliferation of LA-N-5 cells cultured with different concentrations of matrine. MYCN gene mRNA expression in LA-N-5 cells was measured using real time RT-PCR with SYBR GREEN I fluorescence.</p><p><b>RESULTS</b>The proliferation of LA-N-5 cells was obviously inhibited by matrine in a dose- and time- dependent manner. Matrine of 1.00 mg/mL treatment for 72 hrs produced a best effect, with an inhibitory rate of LA-N-5 cell proliferation of 36.3% and an inhibitory rate of MYCN gene mRNA expression of 44.6%.</p><p><b>CONCLUSIONS</b>Matrine may inhibit the growth of neuroblastoma cells and down-regulate MYCN mRNA expression. It may be promising as a new drug for treatment of neuroblastoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Quinolizines
/
RNA, Messenger
/
Nuclear Proteins
/
Oncogene Proteins
/
Cell Line, Tumor
/
Cell Proliferation
/
Drug Therapy
/
Alkaloids
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2008
Type:
Article
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