Study on qualitative and quantitatire methods for Ceratostigma willmottianum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the qualitative and quantitative methods of Caratostigma willmottianum.</p><p><b>METHOD</b>The roots of Radix Ceratostigmae were identified by TLC with the authentic medicinal material and plumbagin as the reference. The contents of plumbagin in Radix Ceratostigmae were determined by HPLC.</p><p><b>RESULT</b>The TLC method was simple and specific. A Diamonsil C18 column was used. The mobile phase was methanol-water (7525). Plumbagin in the sawple extract was separated well. The linear range of plumbagin was 33.6-313.6 ng. The average recovery of plumbagin was 100.3% and RSD was 1.9%.</p><p><b>CONCLUSION</b>The methods can be used for identification and determination of plumbagin in C. willmottianum.</p>