Studies on HPLC fingerprint of co-hirudo injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1825-1827, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-252217
ABSTRACT
<p><b>OBJECTIVE</b>Using HPLC To determine hypoxanthine in co-hirudo injection for establishing its HPLC fingerprint, and evaluating its internal quality.</p><p><b>METHOD</b>The chromatographic separation was performed on a Kromasil C18 column (4.6 mm x 250 mm,5 microm). A linear gradient elution with A (0.01 mol x L(-1) x KH2PO4) and B (50% methanol) was used, the flow rate was 0.8 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was at normal.</p><p><b>RESULT</b>Hypoxanthine was used as the reference substance in the fingerprint of co-hirudo injection, it showed 15 common peaks and theirs similarity threshod was 0.97.</p><p><b>CONCLUSION</b>This method was accurate, repeatable and useful for the quality control of co-hirudo injection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Hypoxanthine
/
Leeches
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article
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