Level of DNA adduct in lung cancer tissues and its influencing factors / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>To detect DNA adduct in lung cancer tissues and analyze the factors influencing formation of DNA adduct.</p><p><b>METHODS</b>DNA was extracted from the tumor tissues derived from 34 patients with lung cancer. DNA adducts were analyzed using 32P-postlabeling method with P 1 nuclear modification.</p><p><b>RESULTS</b>DNA adduct level was significantly higher in smoking patients than that in non smoking patients(P < 0.05). DNA adduct level was related to the histological classification, degree of cell differentiation and lymph node metastasis, but not to age and sex.</p><p><b>CONCLUSIONS</b>DNA adduct level might be used as a potential marker to estimate malignant degree for lung cancer.</p>