Expression profile of 60 lung cancer related genes in BEP2D cell and R15Hp35T-2 cell / 中国肺癌杂志
Chinese Journal of Lung Cancer
;
(12): 321-325, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-252426
ABSTRACT
<p><b>BACKGROUND</b>To profile the expression patterns of 60 lung cancer related genes in human bronchial epithelial cell (BEP2D) and alpha-particle induced malignantly transformed cell (R15Hp35T-2).</p><p><b>METHODS</b>Sixty lung cancer related cDNAs were micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cell and R15Hp35T-2 cell was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA.</p><p><b>RESULTS</b>Compared with the BEP2D cell, 27 genes up-regulated and 7 down-regulated in the R15Hp35T-2 cell. The expression abundance of most tumor suppressor genes were similar in the two kinds of cells, however, most oncogenes and growth factor genes were overexpressed in R15Hp35T-2 cell.</p><p><b>CONCLUSIONS</b>In malignantly transformed human bronchial epithelial cell model induced by alpha-particle, some oncogenes and growth factor genes may promote the malignant transformation together.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Lung Cancer
Year:
2002
Type:
Article
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