Lingmao recipe inhibits starvation induced autophagy in HepG2.2.15 cell / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 499-503, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-252481
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Lingmao Recipe (LR) on starvation-induced autophagy in HepG 2.2.15 cell.</p><p><b>METHODS</b>Fifteen SD rats were selected to prepare LR drug serum. The Earle's balanced salt solution (EBSS) group, the EBSS + vehicle serum group, the EBSS + 5 times LR serum group, the EBSS + 10 times LR serum group, and the DMEM group were set up, 3 samples in each group. The HepG2.2.15 cells were cultured for 1, 2, 4, and 6 h respectively. The pEGFP-N1-LC3B eukaryotic expression vector was constructed and transfected with HepG2. 2.15 cell. The GFP-LC3B morphological changes were observed under fluorescent microscope. The ratio of the dotty GFP-LC3B number and the GFP-LC3B transfected HepG2.2.15 number was calculated. The intracytoplasmic autophagosome changes were observed using electronic transmission electron. The microtubule-associated protein 1 light chain 3 beta II/I was detected in HepG2.2.15 of each group using Western blot.</p><p><b>RESULTS</b>Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). The electronic transmission electron showed that the formation of autophagosome was inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group. Results of Western blot showed that microtubule-associated protein 1 light chain 3 beta II/I obviously decreased more in the EBSS +10 times LR serum group than in the EBSS +vehicle serum group (P<0.01).</p><p><b>CONCLUSION</b>LR could inhibit starvation-induced autophagy in HepG 2.2.15.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Autophagy
/
Drugs, Chinese Herbal
/
Transfection
/
Rats, Sprague-Dawley
/
Culture Media
/
Serum
/
Hep G2 Cells
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2012
Type:
Article
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