Effects of shikonin on the proliferation and apoptosis of HL-60 cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 239-243, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-252569
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of shikonin for inducing the apoptosis of human promyelocytic leukemia cell HL-60.</p><p><b>METHODS</b>The effects of shikonin on the HL-60 cell proliferation were detected using MTT. The apoptosis rate was analyzed by Annexin-V/PI double staining. The expression level of the bcl-2 gene was detected using semi-quantitative reverse transcriptase PCR (RT-PCR), thus analyzing the correlation between the bcl-2 expression level and the apoptosis of HL-60.</p><p><b>RESULTS</b>Shikonin could inhibit the proliferation of HL-60 cells with the concentration range of 1-8 microg/mL in a time- and concentration-dependent manner. Two microg/mL shikonin could induce the apoptosis of HL-60 cells in a time-dependent manner. The expression level of bcl-2 was obviously down-regulated at 2 microg/mL shikonin.</p><p><b>CONCLUSIONS</b>Shikonin could induce the apoptosis of HL-60 cells. Its mechanism was correlated with down-regulation of the expression level of bcl-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Naphthoquinones
/
Apoptosis
/
HL-60 Cells
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Proliferation
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2012
Type:
Article
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