Expression changes of major outer membrane protein antigens in Leptospira interrogans during infection and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 156-163, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-252650
ABSTRACT
<p><b>OBJECTIVE</b>To determine expression changes of major outer membrane protein(OMP) antigens of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain Lai during infection of human macrophages and its mechanism.</p><p><b>METHODS</b>OmpR encoding genes and OmpR-related histidine kinase (HK) encoding gene of L.interrogans strain Lai and their functional domains were predicted using bioinformatics technique. mRNA level changes of the leptospiral major OMP-encoding genes before and after infection of human THP-1 macrophages were detected by real-time fluorescence quantitative RT-PCR. Effects of the OmpR-encoding genes and HK-encoding gene on the expression of leptospiral OMPs during infection were determined by HK-peptide antiserum block assay and closantel inhibitive assays.</p><p><b>RESULTS</b>The bioinformatics analysis indicated that LB015 and LB333 were referred to OmpR-encoding genes of the spirochete, while LB014 might act as a OmpR-related HK-encoding gene. After the spirochete infecting THP-1 cells, mRNA levels of leptospiral lipL21, lipL32 and lipL41 genes were rapidly and persistently down-regulated (P <0.01), whereas mRNA levels of leptospiral groEL, mce, loa22 and ligB genes were rapidly but transiently up-regulated (P<0.01). The treatment with closantel and HK-peptide antiserum partly reversed the infection-based down-regulated mRNA levels of lipL21 and lipL48 genes (P <0.01). Moreover, closantel caused a decrease of the infection-based up-regulated mRNA levels of groEL, mce, loa22 and ligB genes (P <0.01).</p><p><b>CONCLUSION</b>Expression levels of L.interrogans strain Lai major OMP antigens present notable changes during infection of human macrophages. There is a group of OmpR-and HK-encoding genes which may play a major role in down-regulation of expression levels of partial OMP antigens during infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Outer Membrane Proteins
/
Virulence
/
Cell Line
/
Chaperonin 60
/
Allergy and Immunology
/
Genetics
/
Leptospira interrogans
/
Lipoproteins
/
Macrophages
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS