Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 61-66, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-252664
ABSTRACT
<p><b>OBJECTIVE</b>To determine 5-lipoxygenase (5-LOX) expression and the effect of zileuton, a selective 5-LOX inhibitor,on hippocampal neuron injury induced by global cerebral ischemia in rats.</p><p><b>METHODS</b>Global cerebral ischemia was induced by bilateral common carotid artery occlusion combined with hypotension in rats. 5-LOX expression was detected by Western blot analyses and 5-LOX localization was visualized by immunohistochemistry and double immunofluorescence methods. The 5-LOX inhibitor zileuton (10, 30, 50 mg/kg) was orally administered for 3 d after ischemia.</p><p><b>RESULTS</b>The 5-LOX expression was increased in the ischemic hippocampus on d1-7 (peaked at d3), and 5-LOX protein was primarily localized in neurons and translocated to the nuclei in the hippocampal CA1 region after ischemia. The 5-LOX inhibitor zileuton (30, 50 mg/kg) reduced ischemia-induced hippocampal neurons death 3d after ischemia.</p><p><b>CONCLUSION</b>5-LOX is involved in global cerebral ischemic damage in rats, and the 5-LOX inhibitor zileuton has a protective effect on neuronal damage in the rat hippocampus following global cerebral ischemia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Arachidonate 5-Lipoxygenase
/
Brain Ischemia
/
Lipoxygenase Inhibitors
/
Rats, Sprague-Dawley
/
Disease Models, Animal
/
CA1 Region, Hippocampal
/
Hydroxyurea
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2013
Type:
Article
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