Roles of NRF2 regulating gamma-glutamylcysteine synthetase in lung of rats with chronic obstructive pulmonary disease / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 339-342, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-252774
ABSTRACT
<p><b>AIM</b>To investigate the expression and relationship of gamma-glutamylcysteine synthetase (gamma-GCS) and NF-E2-related factor2 (NRR2) in lung of rat with chronic obstructive pulmonary disease (COPD)in order to elucidate the possible important role of gamma-GCS and NRF2 in pathogenesis of COPD.</p><p><b>METHODS</b>The rat COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposed to cigarette smoke daily. The gamma-GCS activity was measured, the expression of gamma-GCS mRNA in lung was examined by in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR), the protein expressions of NRF2, gamma-GCS in lung were detected by immunohistochemical (IH) and Western blot respectively.</p><p><b>RESULTS</b>The gamma-GCS activity was higher in COPD group than that in control group. The expressions of gamma-GCS mRNA in COPD group was stronger than those in control group. ISH showed that the gamma-GCS mRNA was expressed in alveolar epithelium and bronchial smooth muscle cell in COPD. The protein expressions of NRF2, gamma-GCS were significantly higher than the control group. IH showed that NRF2, gamma-GCS proteins were expressed in alveolar and bronchial epithelium in the COPD group. There was a positive correlation between NRF2 and gamma-GCS and gamma-GCS mRNA.</p><p><b>CONCLUSION</b>NRF2 may play an important role in the mechanism of COPD oxidative stress vis up-regulation of gamma-GCS.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Random Allocation
/
Rats, Wistar
/
Oxidative Stress
/
Pulmonary Disease, Chronic Obstructive
/
NF-E2-Related Factor 2
/
Glutamate-Cysteine Ligase
/
Lung
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2008
Type:
Article
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