Red clover isoflavones inhibit the proliferation and promote the apoptosis of benign prostatic hyperplasia stromal cells / 中华男科学杂志
National Journal of Andrology
;
(12): 34-39, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-252877
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia (BPH) stromal cells.</p><p><b>METHODS</b>We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5, 25, 50 and 100 microg/ml, and established a PBS blank control, a dimethyl sulphoxide (DMSO) negative control and four finasteride positive control groups (at the concentration of 12.5, 25.0, 50.0 and 100.0 microg/ml). We determined the effects of different concentrations of red clover isoflavones on the proliferation of the cells by MTT assay and on their apoptosis by Annexin V/PI double staining flow cytometry.</p><p><b>RESULTS</b>Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 microg/ml, compared with 5.17% in the blank control group (P < 0.05), and more obviously at a higher concentration. At 50.0 microg/ml, red clover isoflavones exhibited a weaker inhibitory effect than finasteride (28% vs 69.88% , P < 0.05). Annexin V/PI double staining flow cytometry showed that red clover isoflavones at 25.0 microg/ml induced the apoptosis of the prostate stromal cells by (18.54 +/- 2.5)%, with significant differences from the negative control and blank control (P < 0.01).</p><p><b>CONCLUSION</b>Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostate
/
Prostatic Hyperplasia
/
Plant Extracts
/
Cells, Cultured
/
Chemistry
/
Stromal Cells
/
Apoptosis
/
Trifolium
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2010
Type:
Article
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