Rapid analysis of psoralen and isopsoralen in Psoralea corylifolia by microwave-assisted extraction / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a convenient and rapid method of microwave-assisted extraction (MAE) for the extraction of psoralen and isopsoralen in Psoralea corylifolia.</p><p><b>METHOD</b>The effects of various parameters on the MAE were investigated with several single-factor tests, and compared with the traditional extraction.</p><p><b>RESULT</b>The optimal conditions of MAE were as follows the extractine time was 3 min, and the ratio of solvent to material was 400 mL x g(-1) by using methanol as the solvent. Compared with soxhlet extraction, heat reflux extraction, and ultrasound-assisted extraction, MAE needed only 3 min to give the highest yield of psoralen and isopsoralen, while the other extraction methods needed several hours and gave lower yield.</p><p><b>CONCLUSION</b>The proposed method has greatly shortened extraction time and improved extraction yield, so it provides a neotype, simple and rapid measure for the separation of psoralen and isopsoralen from P. corylifolia.</p>