Simultanenous determination of contents of four nitrogenous compounds in Pinellia peda-tisecta by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a HPLC method for the quantitative determination of four nitrogenous compounds in Pinellia peda-tisecta.</p><p><b>METHOD</b>The determination was carried out on Diamonsil-C18 column(4. 6 mm x 250 mm, 5 microm) eluted with acetonitrile-water-triethylamine in gradient elution acetonitrile-water- triethylamine. The mobile phase was a flow rate of 1.0 mL x min(-1) and detection wavelength as 254 nm.</p><p><b>RESULT</b>The linear ranges of trigonelline, thymine, amidopurine and hypoxanthine were 0.2 - 1.0 microg (r = 0.999 6), 2.6-13 microg (r = 0.999 8), 0.55-2.75 microg (r = 0.999 9), 0.65-3.25 microg (r = 0.999 8), respectively. The average recoveries of trigonelline, thymine, amidopurine and hypoxanthine were 100.6%, 99.89%,100.5% and 100.0% respectively, and the RSD of the above four compounds were 2.4%, 0.20%, 1.1%, 1.4%, respectively.</p><p><b>CONCLUSION</b>This method is reliable, accurate and specific. It can be used for quality control of the tube of P. peda-tisecta.</p>