Tumor necrosis factor-alpha induces release of endothelial microparticles from human endothelial cells / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 1137-1140, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-252997
ABSTRACT
<p><b>OBJECTIVE</b>The present study was designed to investigate whether Tumor necrosis factor (TNF)-alpha stimulates release of endothelial microparticles (EMPs) by human endothelial cells, and whether EMPs may serve as a promising marker for endothelial injury and dysfunction.</p><p><b>METHODS</b>Human umbilical venous endothelial cells (HUVEC) were incubated with or without TNF-alpha for 24 hours at 37 degrees C. EMPs generated on the surface of HUVEC were observed with a scanning electron microscopy. The CD31 and CD51 positive EMPs in culture supernatants were measured by flow cytometer.</p><p><b>RESULTS</b>Fewer vesicles were observed on cell surface of control group, in TNF-alpha-stimulated one, however, cells manifested a blebby surface (eruption phenomenon) and more vesicles on surface were observed. The levels of EMPs were significantly increased in TNF-alpha stimulated cells compared with controls [CD31 + EMP, (164 +/- 63)/1000 cells vs. (42 +/- 10)/1000 cells, P < 0.05; CD51 + EMP, (260 +/- 108)/1000 cells vs. (19 +/- 4)/1000 cells, P < 0.05].</p><p><b>CONCLUSION</b>TNF-alpha can stimulate HUVEC to release EMPs which may serve as a surrogate marker for endothelial injury and dysfunction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Umbilical Veins
/
Endothelium, Vascular
/
Cells, Cultured
/
Tumor Necrosis Factor-alpha
/
Cell Biology
/
Cytoplasmic Granules
/
Endothelial Cells
/
Flow Cytometry
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2005
Type:
Article
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