Atorvastatin upregulates the expression of PPAR alpha/gamma and inhibits the hypertrophy of cardiac myocytes in vitro / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 1080-1084, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-253008
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of atorvastatin on angiotensin II (Ang II)-induced hypertrophy of cardiac myocytes (MC) and the changes of mRNA expression of peroxisome proliferators-activated receptor alpha, gamma (PPAR alpha, gamma) subtypes in vitro.</p><p><b>METHODS</b>Hypertrophy in neonatal rat MC was established with Ang II and treated with atorvastatin. The surface area of MC was analyzed by the aid of NIH Image J software, and the synthetic rate of protein in MC was detected by (3)H-leucine incorporation. mRNA expression of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), matrix metalloproteinase (MMP) 9, MMP2, interleukin1beta (IL-1beta) and PPARalpha, gamma was measured by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Changes of MC were detected induced by Ang II, including increases in surface area, mRNA expression of ANP, BNP, MMP9, MMP2 and IL-1beta, and (3)H-leucine incorporation, as well as a decrease in mRNA expression of PPARalpha, gamma. Treatment with atorvastatin inhibited the changes above in a dose-dependent manner, but no change was found in treated with DMSO.</p><p><b>CONCLUSION</b>Atorvastatin inhibits cardiac hypertrophy in vitro. It is suggested that atorvastatin has a potential role in the prevention and treatment of cardiac diseases such as cardiac hypertrophy, and PPAR alpha and gamma maybe involved in this process.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyrroles
/
Angiotensin II
/
Up-Regulation
/
Cells, Cultured
/
Gene Expression Regulation
/
Rats, Wistar
/
Cardiomegaly
/
Myocytes, Cardiac
/
PPAR alpha
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2005
Type:
Article
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