Expressions of Tau protein during the differentiation process of mesenchymal stem cells into neural cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 419-422, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-253125
ABSTRACT
<p><b>AIM</b>To observe expressions and changes of Tau protein, pSer202 and Tau protein's contents during the differentiation process of bone-marrow mesenchymal stem cells (MSCs) into neural cells, and discuss Tau's effects on it.</p><p><b>METHODS</b>EGF and bFGF were combined for the induction of 4th, 8th, and 12th-MSCs into neural cells. Expressions of Tau protein and pSer202 were tested by immunocytochemistry. ELISA assay was applied for testing Tau protein's contents during differentiation process.</p><p><b>RESULTS</b>Positive rates of Tau protein in uninduced MSCs of 4th, 8th, and 12th-MSCs were under < 6%; After 14-day induction, the cellular morphologic characteristics in different passages were very similar to neurons, positive rates of Tau protein had no significant differences between passages (P > 0.05), but had differences with their uninduced groups (P < 0.05). There hadn't had expression of pSer202 in uninduced and induced groups of passages. ELISA assay indicated that there was an upward tendency in Tau protein's contents during the 14-day induction process, those in the 14th day had no significant differences between passages too (P > 0.05).</p><p><b>CONCLUSION</b>The increase in Tau protein's expressions and its non-phosphorylated state may make for MSCs differentiating into normal neural cells and formation of neuronal processes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Tau Proteins
/
Cell Biology
/
Mesenchymal Stem Cells
/
Guinea Pigs
/
Metabolism
/
Neurons
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2006
Type:
Article
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