Redox modulation of large conductance calcium-activated potassium channels in rat cultured trigeminal ganglion neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 390-393, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-253133
ABSTRACT
<p><b>AIM</b>To observe redox modulation of ion channel in trigeminal ganglion neurons by oxidants and reducing agents.</p><p><b>METHODS</b>The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.</p><p><b>RESULTS</b>Methionine-specific oxidant chloramine-T (Ch-T) 1 mmol/L slightly increased the current amplitude and this enhancement did not antagonized by DTT. In contrast, cysteine-specific reagent 5, 5'-dithio-bis(2-nitrobenzoic acid) (DTNB) 500 micromol/L significantly decreased current amplitude of BK(Ca) channels. The effect was reversed by the reducing agent 2 mmol/L 1, 4-dithio-DL-threitol (DTT).</p><p><b>CONCLUSION</b>Reactive oxygen species were definitely involved in regulation of native neuronal function via redox modulation of BK(Ca) channels, which are suggested to play compensatory roles under oxidative stress-related conditions.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Physiology
/
Cells, Cultured
/
Trigeminal Ganglion
/
Rats, Sprague-Dawley
/
Patch-Clamp Techniques
/
Cell Biology
/
Large-Conductance Calcium-Activated Potassium Channels
/
Metabolism
/
Neurons
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2006
Type:
Article
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