Endothelium-independent vasorelaxation of plant-derived estrogen biochanin A and its mechanism in rat aortic rings / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 274-277, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-253166
ABSTRACT
<p><b>AIM</b>To investigate the mechanisms of vasodilatation of plant-derived estrogen biochanin A.</p><p><b>METHODS</b>Isolated aortic ring preparations from Sprague-Dawley rats were suspended in individual organ baths. The tension was measured isometrically.</p><p><b>RESULTS</b>Biochanin A at the range of 10(-9)-10(-4) mol/L provoked concentration-dependent and endothelium-independent relaxation of the rings constricted by phenylephrine (10(-5) mol/L). Biochanin A caused concentration-dependent relaxation of denuded rings precontracted with KCl (6 x 10(-2) mol/L). Glibenclamide (3 x 10(-6) mol/L), a selective inhibitor of ATP-sensitive potassium channels, and tetraethylammonium (5 x 10(-3) mol/L), a Ca2+ -activated K+ channel inhibitor, significantly attenuated the relaxation induced by biochanin A. The vasoconstriction induced by phenylephrine was decreased by biochanin A in Ca2+ -free medium.</p><p><b>CONCLUSION</b>The endothelium-independent relaxation of thoracic aorta induced by biochanin A might be mediated by ATP-sensitive K+ channels, Ca2+ -activated K+ channels and intracellular Ca2+ release from sarcoplasmic reticulum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Thoracic
/
Pharmacology
/
Physiology
/
Vasodilation
/
In Vitro Techniques
/
Rats, Sprague-Dawley
/
Genistein
/
KATP Channels
/
Metabolism
/
Muscle, Smooth, Vascular
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2006
Type:
Article
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