Gamma-globin synthesis in K562 cells induced with Tortois plastron, Astragali, Salviae miltiorrhizae and Codonopsis pilosulae / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 520-524, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-253284
ABSTRACT
This study was purposed to investigate the effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on gamma-globin gene synthesis in K562 cells in vitro. Benzidine staining was used to clarify the dose-and time-dependent effects of tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae on hemoglobin synthesis in K562 cells and Western blotting was performed to determine the level of hemoglobin F (alpha(2)gamma(2)). The results indicated that the K562 cells treated with 4 kinds of traditional Chinese medicine had different stain rates of benzidine for 23.5% (tortois plastron), 19.8% (astragali), 15.8% (salviae miltiorrhizae) and 14.5% (codonopsis pilosulae) at 6 days after the treatment. Western blot indicated that synthesis of HbF increased. It is concluded that tortois plastron, astragali, salviae miltiorrhizae and codonopsis pilosulae enhance globins-gamma synthesis level and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Materia Medica
/
Chemistry
/
Rats, Sprague-Dawley
/
K562 Cells
/
Codonopsis
/
Astragalus propinquus
/
Salvia miltiorrhiza
/
Gamma-Globins
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
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