CMV pp65 gene modified dendritic cells activate autologous T cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 397-400, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-253311
ABSTRACT
Cytomegalovirus (CMV) infection is a dangerous complication in patients with chronic graft versus host disease (cGVHD). CMV-specific immunity depends on the activity of T cells. This study was aimed to investigate the effect of CMV pp65 gene modified dendritic cells (DCs) on activation of autologous T cells. Lentivirus system was utilized to introduce the CMV full-length pp65 gene into mouse DCs; CpG-DNA was used to induce mature DCs; flow cytometry and immunofluorescence were used to determine the expression of antigen and IFNgamma in T lymphocytes. The results showed that the DCs were infected with lentivirus at a multiplicity of infection (MOI) of 50 with optimal infectious efficiency of 30%-40%; mature DCs expressing pp65 gene could stimulate autologous naive T cells to express CD69 specifically; mature DCs expressing PP65 could stimulate autologous CD4+ or CD8+ T cells to produce IFNgamma. It is concluded that CMV pp65-modified and CpG-DNA-induced mature DCs can activate CMV-specific T lymphocytes in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphoproteins
/
Dendritic Cells
/
DNA
/
CD4-Positive T-Lymphocytes
/
Antigens, Differentiation, T-Lymphocyte
/
Antigens, CD
/
Viral Matrix Proteins
/
Interferon-gamma
/
Lentivirus
/
CD8-Positive T-Lymphocytes
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS