Foxp3-transfected CD4+ CD25- T cells suppress function of dendritic cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 164-169, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-253359
ABSTRACT
To explore the relationship between expression of Foxp3 gene and immune activity of CD4(+) T cells, the Foxp3 gene was transfected with retroviral vector and applied to forcedly express Foxp3 protein in naive CD4(+)CD25(-) T cells, and then the effect of transfected CD4(+)CD25(-) T cells on immune co-stimulatory molecules and immune function of dendritic cells (DCs) was investigated, and the dependence of direct contact between Foxp3-transfected CD4(+)CD25(-) T cells and DCs was clarified by Transwell test. The results showed that through transfection of retroviral vector, CD4(+)CD25(-) T cells model expressing Foxp3 was established. At 1 week after transfection, proportion of T cells expressing Foxp3 was 38%. CD4(+)CD25(-) T cells forcedly expressing Foxp3 could play immune suppression role in vitro and induce down-regulation of CD80 and CD86 expression on the membrane of DCs. The lymphocyte proliferation test in vitro indicated that Foxp3 transfected CD4(+)CD25(-) T cells could inhibit effect of DCs on activation of allo-lymphocytes. It is concluded that the effect of Foxp3-transfected CD4(+)CD25(-) T cells on DC depends on intercellular direct contact.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retroviridae
/
Dendritic Cells
/
Transfection
/
CD4-Positive T-Lymphocytes
/
CD4 Antigens
/
T-Lymphocytes, Regulatory
/
Allergy and Immunology
/
Forkhead Transcription Factors
/
Interleukin-2 Receptor alpha Subunit
/
Genetic Vectors
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS