Effect of co-culture of bone marrow mesenchymal stem cells and K562 cells on expression of interleukin 8 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 156-158, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-253361
ABSTRACT
To investigate the effects of interaction between human bone marrow mesenchymal stem cells (MSCs) and K562 cells on the expression of proangiogenic factor IL-8, the K562 cells were co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium while the controlled K562 cells were cultured alone. The IL-8 gene expression of K562 cells and MSCs was determined by RT-PCR. The results indicated that the expression of IL-8 in K562 cells when co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium was obviously higher than that of K562 cells cultured alone (p<0.01). MSCs co-cultured with K562 cells also had an increased level of IL-8 compared with MSCs cultured alone (p<0.01). It is concluded that the interaction between MSCs and K562 cells via direct contact and the cytokine network promotes expression of IL-8.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Bone Marrow Cells
/
Interleukin-8
/
Coculture Techniques
/
K562 Cells
/
Cell Biology
/
Mesenchymal Stem Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
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