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Comparative characterization of mesenchymal stem cells from human umbilical cord tissue and bone marrow / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 140-146, 2008.
Article in Chinese | WPRIM | ID: wpr-253364
ABSTRACT
This study was purposed to compare the biological characteristics of umbilical cord-derived mesenchymal stem cells (UC-MSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs). The frequency of successful isolation, cell yield, colony-forming units-fibroblastics (CFU-F), proliferation capacity, immunophenotype and multi-differentiation potentials of UC-MSCs and BM-MSCs were determined by limiting dilution assay, flow cytometry, invert microscopy, RT-PCR and so on, the determined results were compared. The results showed that MSCs were successfully isolated from all the 36 portion of UC tissue and 8 portion of BM. Although the mean number of nucleated cells isolated from UC tissue was significantly lower than that from BMs (1 x 10(6)/cm vs 5.5 x 10(7)/ml) (p=0.0002), no significant differences of the yield of adherent cells were observed (8.6 x 10(5)/cm vs 8.4 x 10(5)/ml) (p>0.05). UC-MSCs shared the most of the characteristic of BM-MSC, including fibroblastic-like morphology, typical immunophenotype, cell cycle status, adipogenic and osteogenic differentiation potentials. However, the CFU-F frequency was higher in UC (11609+/-0.18) than that in BM (135700+/-0.01) (p<0.05). Furthermore, the proliferation capacity of UC-MSCs was higher than that of BM-MSCs; the expressions of CD106 and HLA-class I in UC-MSCs were lower than those in BM-MSCs (p<0.05). It is concluded that the cell yield and most biological characteristics of UC-MSCs are similar to BM-MSCs, but UC-MSCs possess the higher proliferation capacity, and the lower expression of HLA-class I and HLA-DR as compared with BM-MSCs, therefore the human umbilical cord tissue may be considered as a promising alternative to bone marrow as a source of MSCs.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Organ Specificity / Physiology / Umbilical Cord / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Cell Biology / Mesenchymal Stem Cells Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Organ Specificity / Physiology / Umbilical Cord / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Cell Biology / Mesenchymal Stem Cells Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article