Effects of scorpion venom heat-resistant protein on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 315-318, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-253413
ABSTRACT
<p><b>AIM</b>To investigate the effects of scorpion venom heat-resistant protein (SVHRP) on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons.</p><p><b>METHODS</b>We observed morphological changes, celluar vigor, NPY-immunoreactivity and NPY mRNA expression by means of Thionine staining, MTT assay, immunocytochemistry and RT-PCR, respectively, on the primitively cultured Sprague-Dawley rat hippocampal neuron treated with KA and SVHRP for 24 h.</p><p><b>RESULTS</b>MTT assay and morphologic analysis showed that SVHRP markedly increased neuron survival-rate, and protected them from kA-induced damage. The expression of NPY-immunoreactivity and NPY mRNA in SVHRP group increased obviously compared with other groups.</p><p><b>CONCLUSION</b>SVHRP protected the primitively cultured hippocampal neurons from KA-induced neuroexcitotoxicity and promoted the expression of NPY.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Scorpion Venoms
/
Neuropeptide Y
/
Cells, Cultured
/
Cell Death
/
Rats, Sprague-Dawley
/
Cell Biology
/
Hippocampus
/
Kainic Acid
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2007
Type:
Article
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