Effects of ginkgo biloba extracts on NMDA-activated currents in acutely isolated rat hippocampal neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 305-308, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-253416
ABSTRACT
<p><b>AIM</b>To investigate effect of ginkgo biloba extract (GBE) on N-methyl-D-aspartate (NMDA)-activated currents (I(NMDA)) and evaluate further the modulatory effects of Micro-GBE/Nano-GBE.</p><p><b>METHODS</b>By means of whole-cell patch clamp technique, NMDA-activated currents from acutely isolated rat hippocampal neurons were recored.</p><p><b>RESULTS</b>The majority of the neurons examined (81.8%, 90/110) were sensitive to NMDA (1 mmol/L) and its co-agonist Gly (10 micromol/L). NMDA activated an inward current, which manifested apparent desensitization and could be blocked by its specific antagonist MK-801. After the neurons were treated with Micro/Nano GBE (0.1 mg/ml) followed by the application of NMDA (1 mmol/L) and Gly (10 micromol/L) for 30 s, it was show that NMDA-activated currents were obviously inhibited (P < 0.01, n = 8). The inhibitory rate were 40% +/- 17% and 64% +/- 15% respectively. It showed that the modulatory effect of Nano-GBE (dissolved in the stander extracellular solution) on NMDA-activated current was significantly higher than that of Micro-GBE (dissolved in DMSO) (P < 0.05).</p><p><b>CONCLUSION</b>The inward currents activated by NMDA could be depressed by Micro-GBE and Nano-GBE. The modulatory effects of GBE on NMDA-activated current are expected to contribute to the neuroprotective effects of ginkgo biloba extracts. In addition, at the same concentration, the modulatory effect of Nano-GBE on NMDA-activated current is better than that of Micro-GBE.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Plant Extracts
/
Cells, Cultured
/
N-Methylaspartate
/
Rats, Sprague-Dawley
/
Receptors, N-Methyl-D-Aspartate
/
Patch-Clamp Techniques
/
Ginkgo biloba
/
Hippocampus
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2007
Type:
Article
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