Effects of CGRP on LPS-induced MMP-9 secretion by alveolar macrophages / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 211-215, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-253443
ABSTRACT
<p><b>AIM</b>To explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro.</p><p><b>METHODS</b>The supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography.</p><p><b>RESULTS</b>(Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The inhibiting effects of CGRP were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (p < 0.05).</p><p><b>CONCLUSION</b>These data suggested that CGRP involved in the MMP-9 secretion by AM, partly, via PKC and CaM pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Lipopolysaccharides
/
Macrophages, Alveolar
/
Rats, Wistar
/
Receptors, Calcitonin Gene-Related Peptide
/
Bodily Secretions
/
Matrix Metalloproteinase 9
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2007
Type:
Article
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