Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1099-1104, 2005.
Article
in English
| WPRIM
| ID: wpr-253523
ABSTRACT
<p><b>AIM</b>To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel.</p><p><b>METHODS</b>Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs).</p><p><b>RESULTS</b>Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively.</p><p><b>CONCLUSION</b>Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Breast Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Cell Cycle
/
Paclitaxel
/
Apoptosis
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Cell Line, Tumor
Limits:
Female
/
Humans
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2005
Type:
Article
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