Effects of dipfluzine on expressions of E-selectin, P-selectin, and ICAM-1 in brain ischemia-reperfusion rats / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1091-1095, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-253525
ABSTRACT
<p><b>AIM</b>To evaluate the effects of dipfluzine on the expressions of E-selectin, P-selectin, and ICAM-1 and the infiltration of polymorphonuclear leukocytes in brain ischemia-reperfusion rats.</p><p><b>METHODS</b>The model of focal cerebral ischemia was established with the Zea-Longa occluding suture. Dipfluzine (0.25, 0.5 and 1 mg x kg(-1)), flunarizine 0.5 mg x kg(-1) and solvent were injected separately into lingual vein at 30 min after ischemia. The occluding suture was slowly taken away to cause reperfusion at 1 h after ischemia. Rats were decapitated under anesthesia at 24 h after ischemia-reperfusion and brains were immediately removed to do the following procedures. Effects of dipfluzine on morphology of the brain tissue were observed through hematoxylin-eosin (HE) staining. By immunohistochemistry and flow cytometry technique and biochemical method, effects of dipfluzine on P-selectin, E-selectin, ICAM-1 and myeloperoxidase (MPO) were observed.</p><p><b>RESULTS</b>Dipfluzine could relieve pathological damages in the brain tissue after ischemia-reperfusion, and reduce the expressions of E-selectin, P-selectin and ICAM-1 and activities of MPO in dose-dependent manner.</p><p><b>CONCLUSION</b>Dipfluzine depresses the expressions of P-selectin, E-selectin, and ICAM-1, which are correlated with their effects on the activities of MPO, suggesting that dipfluzine has anti-inflammation effect in certain extent and could protect brain tissue from ischemia-reperfusion injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Reperfusion Injury
/
Cerebral Cortex
/
Brain Ischemia
/
Cinnarizine
/
Rats, Sprague-Dawley
/
Peroxidase
/
Neuroprotective Agents
/
Intercellular Adhesion Molecule-1
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2005
Type:
Article
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