Analysis of ephedrine and its metabolites in rat urine by HPLC-ESI-ITMSn / 药学学报
Acta Pharmaceutica Sinica
; (12): 838-841, 2005.
Article
in Zh
| WPRIM
| ID: wpr-253557
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To estabilish a rapid and sensitive LC-ESI-ITMSn method for the identification of ephedrine and its main metabolites in rat urine.</p><p><b>METHODS</b>After optimizing the detection condition of LC-ESI-ITMSn chromatography and mass spectrometry by using a standard ephedrine, the ionization and cleavage rules of ephedrine in ESI-MS and ESI-MSn modes were summarized, and then serving as the basis for the metabolite analysis of ephedrine in rat urine. Rat urine samples of 0-48 h were collected after ig 10 mg x kg(-1) ephedrine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-ESI-ITMSn.</p><p><b>RESULTS</b>The structures of ephedrine metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, three phase I metabolites and the parent drug ephedrine were identified existing in rat urine, but no phase II metabolites were found.</p><p><b>CONCLUSION</b>The LC-ESI-ITMSn method is rapid and highly sensitive and sepecific, it is suitable for the identification of ephedrine and its metabolites in rat urine.</p>
Full text:
1
Index:
WPRIM
Main subject:
Urine
/
Chemistry
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Chromatography, High Pressure Liquid
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Sensitivity and Specificity
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Rats, Wistar
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Spectrometry, Mass, Electrospray Ionization
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Ephedrine
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Metabolism
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Methods
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Molecular Weight
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2005
Type:
Article