Colony-stimulating factor-1 receptor in rat dental follicle cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 435-437, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-253679
ABSTRACT
<p><b>OBJECTIVE</b>To study localization and expression of CSF-1 receptor protein, in order to discover the CSF-1 and IL-1alpha effects on CSF-1 receptor mRNA levels and to determine if the autocrine effect is inhibited through the CSF-1 receptor.</p><p><b>METHODS</b>Immunolocalization of CSF-1 receptor in the cultured dental follicle cells and in mandibles of the post-natal rats from day 1 to 11 were performed. The effects of different concentrations of CSF-1, IL-1alpha on CSF-1 receptor gene expression were detected by means of RT-PCR.</p><p><b>RESULTS</b>Cultured dental follicle cells were immunostained for the CSF-1 receptor. In vivo, immunostaining showed that the CSF-1 receptor was present in the dental follicle of the first mandibular molar at early post-natally and was either absent or greatly reduced by day 11 post-natally. High concentrations of cvCSF-1 reduced the gene expression of the CSF-1 receptor. IL-1alpha had no effects on CSF-1 receptor mRNA levels.</p><p><b>CONCLUSIONS</b>The expression of CSF-1 receptor reaches a peak early post-natally in the dental follicle of the first mandibular molar of the rat and then subsequently declines. High concentrations of CSF-1 inhibits the expression of CSF-1 receptor, IL-1alpha has no effect on the expression of CSF-1 receptor mRNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Immunohistochemistry
/
Cells, Cultured
/
Chemistry
/
Macrophage Colony-Stimulating Factor
/
Interleukin-1
/
Rats, Sprague-Dawley
/
Receptor, Macrophage Colony-Stimulating Factor
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2003
Type:
Article
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