Influence of enterococci on human sperm membrane in vitro / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 77-81, 2007.
Article
in English
| WPRIM
| ID: wpr-253799
ABSTRACT
<p><b>AIM</b>To study the influence of enterococci on human sperm membrane in vitro.</p><p><b>METHODS</b>Ejaculated human sperm were artificially infected with beta-hemolytic or non-beta-hemolytic enterococci at the bacteria sperm ratio of 501 at 37 degrees . Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling (HOS) test and electron microscopy.</p><p><b>RESULTS</b>Sperm infected with beta-hemolytic enterococci had lower HOS scores compared with non-beta-hemolytic strains or uninfected control (P < 0.01). The HOS test scores of sperm infected with beta-hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non-beta-hemolytic strains showed no significant difference in swelling rate, compared with the control group (P > 0.05). It was shown by electron microscopy that beta-hemolytic enterococci caused significant rupture of human sperm membrane.</p><p><b>CONCLUSION</b>Beta-hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphatidylcholines
/
Physiology
/
Reference Values
/
Spermatozoa
/
Cell Membrane
/
Enterococcus
/
Ejaculation
/
Feces
/
Microbiology
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS