Control of angiogenesis by inhibitor of phospholipase A2 / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 6-12, 2004.
Article
in En
| WPRIM
| ID: wpr-254037
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential effects of angiogenic process by secretory phospholipase A2 (sPLA2) inhibitor-HyPE (linking N-derivatized phosphatidyl-ethanolamine to hyaluronic acid) on human bone marrow endothelial cell line (HBME-1).</p><p><b>METHODS</b>In order to examine the suppressing effects of HyPE on HBME-1 proliferation, migration, and capillary-like tube formation, HBME-1 were activated hy angiogenic factor, specifically by basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), and oncostatin M (OSM) (at a final concentration of 25, 20, and 2.5 ng/mL, respectively), then HBME-1 proliferation, migration, and tube formation were studied in the absence or presence of HyPE. HBME-1 tube formation was specially analyzed in fibrin gel.</p><p><b>RESULTS</b>HyPE effectively inhibited HBME-1 proliferation and migration as a dose-dependent manner, whatever HBME-1 were grown in the control culture medium or stimulated with b-FGF, VEGF, or OSM. In fibrin, the formations of HBME-1 derived tube-like structures were enhanced by all angiogenic factors, but these were strongly suppressed by HyPE.</p><p><b>CONCLUSIONS</b>The results support the involvement of sPLA2 in angiogenesis. It is proposed that sPLA2 inhibitor introduces a novel approach in the control of cancer development.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Peptides
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Pharmacology
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Phosphatidylethanolamines
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Phospholipases A
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Capillaries
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Bone Marrow Cells
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Cell Division
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Cell Movement
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Cells, Cultured
Limits:
Humans
Language:
En
Journal:
Chinese Medical Sciences Journal
Year:
2004
Type:
Article