Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat / 中国医学科学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome.</p><p><b>RESULTS</b>A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein.</p><p><b>CONCLUSION</b>There might be a multigene family of ALR in rat.</p>