HBV transinfected enhances the PD-L expression of cytokines stimulating / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 333-335, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-254066
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the PD-L expression in the liver cell lines transinfected with HBV (HepG2.2.15 cells) can be up-regulated after cytokines stimulating.</p><p><b>METHODS</b>To apply the liver cell lines (HepG2 cells and HepG2.2.15 cells) as a model, the cells were stimulated with IL-4, IFN-alpha and IFN-gamma (final concentration were 10 ng/ml, stimulated for 12 hours) and RT-PCR was carried out to determine the PD-L expression before and after cytokines stimulating.</p><p><b>RESULTS</b>Whether or not transinfected with HBV, IFN-alpha and IFN-gamma both can induce the liver cell lines (HepG2 cells and HepG2.2.15 cells) PD-L1 expression while IL-4 can not; IL-4, IFN-alpha, IFN-gamma all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-gamma can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV.</p><p><b>CONCLUSION</b>IFN-alpha, IFN-gamma both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Virology
/
Gene Expression
/
Hepatitis B virus
/
Cytokines
/
Hepatocytes
/
Cell Line, Tumor
/
Genetics
/
Hepatitis B
/
Liver
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article
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