Dynamic variation of HIV-1 CRF07_BC env gene in early HIV-1 infection / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 290-292, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-254078
ABSTRACT
<p><b>OBJECTIVE</b>To understand the evolution of HIV-1 CRF07_BC envelope, we performed a longitudinal study on two patients during their early HIV-1 infection.</p><p><b>METHODS</b>RNA was extracted from the plasma of the individuals and the C2-C5 fragments of the gp120 gene of HIV-1 were amplified by RT-PCR. Purified DNA segments were inserted into T easy vector and transformed into E. coli Top 10 competent cells. Positive clones were identified by blue-white screening, confirmed by PCR and sequenced by ABI 3700.</p><p><b>RESULTS</b>The samples were collected from the patients every 6 months from seroconversion time. The genetic diversity and divergence in env gene showed consistent increases over time. Our sequence analysis also revealed obvious non-synonymous change in env C1, C3 and V4 regions among these samples.</p><p><b>CONCLUSION</b>The results support the concept that the consistent pattern of viral evolution existed during early phase of HIV-1 infection. C1, C3 and V4 region of env gene may be mainly immunological target during AIDS progression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Variation
/
Virology
/
HIV Infections
/
Longitudinal Studies
/
HIV-1
/
Env Gene Products, Human Immunodeficiency Virus
/
Genetics
Type of study:
Observational study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article
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