Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 287-289, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-254079
ABSTRACT
<p><b>OBJECTIVE</b>To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.</p><p><b>METHODS</b>TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.</p><p><b>RESULTS</b>TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.</p><p><b>CONCLUSION</b>HBX protein could be induced into mouse liver by TAT induced peptide.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Recombinant Fusion Proteins
/
Gene Expression
/
Trans-Activators
/
Cell Membrane
/
Protein Transport
/
Green Fluorescent Proteins
/
Escherichia coli
/
Tat Gene Products, Human Immunodeficiency Virus
/
Viral Regulatory and Accessory Proteins
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article
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