Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.</p><p><b>METHODS</b>Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.</p><p><b>RESULTS</b>The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.</p><p><b>CONCLUSION</b>Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.</p>