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Genotype 1 JEV was isolated again from Liaoning Province, China, 2007 / 中华实验和临床病毒学杂志
Article in Chinese | WPRIM | ID: wpr-254134
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.</p><p><b>METHODS</b>Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.</p><p><b>RESULTS</b>Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.</p><p><b>CONCLUSION</b>Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Phylogeny / Virology / Brain / Female / Male / RNA, Viral / Molecular Sequence Data / Cell Line / China Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Phylogeny / Virology / Brain / Female / Male / RNA, Viral / Molecular Sequence Data / Cell Line / China Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2008 Type: Article