Growth inhibitory effect of adriamycin conjugated to single-chain antibody on human lung adenocarcinoma in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 718-721, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-254262
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the growth inhibitory effect of adriamycin (ADM) conjugated to an anti-lung cancer single-chain antibody (ScFv) 2A7-1 on lung adenocarcinoma cell line A2 in vitro.</p><p><b>METHODS</b>2A7-1 cell culture medium was concentrated by ultra-filtration (with Amicon P10Z filter), and soluble ScFv was purified using RPAS purification kit. ADM was conjugated to 2A7-1 by glutaraldehyde. A(280) and A(490) of the conjugate 2A7-1-ADM were determined by spectrophotometry and the molar ratio of 2A7-1 to ADM was calculated. Immunoreactivity of the conjugate was detected by immunohistochemistry. Its growth inhibitory effect on lung adenocarcinoma cell line A2 was determined by colony formation assay in vitro.</p><p><b>RESULTS</b>The molar ratio of 2A7-1 to ADM was 13.2. The conjugate strongly reacted with A2 cell. Its growth inhibitory effect on A2 cells was 4 times as potent as ADM.</p><p><b>CONCLUSION</b>Adriamycin conjugated to anti-lung cancer single-chain antibody 2A7-1 has much higher cytotoxic activity than unconjugated adriamycin against human lung adenocarcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Immunoglobulin Variable Region
/
Adenocarcinoma
/
Doxorubicin
/
Chemistry
/
Colony-Forming Units Assay
/
Immunoconjugates
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2004
Type:
Article
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