Expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis / 中华肿瘤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the expressions and activities of Rho GTPases in hypoxia and its relationship with tumor angiogenesis.</p><p><b>METHODS</b>Three tumor cell lines were used in this study gastric cancer cell lines AGS, SGC7901 and hepatocellular carcinoma cell line HepG2. Expression level of Rac1 mRNA was detected by semi-quantitative RT-PCR. Activity of Rac1 was determined by pull-down assay and expression of HIF-1alpha, VEGF, p53 and PTEN protein was detected by Westernblot.</p><p><b>RESULTS</b>The expression level of Rac1 mRNA was significantly increased in hypoxia compared to normoxia. Pull-down assay showed that hypoxia-induced activity of Rac1 was elevated in a time-dependent manner and climaxed at 3 hours. The expressions of HIF-1alpha and VEGF protein were up-regulated, while those of PTEN and p53 protein were down-regulated.</p><p><b>CONCLUSION</b>These results indicate that hypoxia enhances Rac1 expression which might be involved in tumor angiogenesis by reacting with hypoxia-responsive genes.</p>